Abstract
BackgroundPrevious studies demonstrated the EGF-targeted phagemid particles carrying siRNA against Akt could be expressed efficiently in the presence of hydroxycamptothecin (HCPT). However, no significant cell growth inhibition was obtained. This study was to further investigate whether the EGF-targeted phagemid particles carrying siRNA would be a promising tool for anti-cancer siRNA delivery.ResultsWe found that pSi4.1-siFAK phagemid particles could significantly inhibit the expression of focal adhesion kinase in the HCPT-treated cells. Moreover, we also observed that the particles could potently suppress cell growth and cell invasion.ConclusionThese results indicated that EGF-targeted phagemid particles might be a promising tool for anti-cancer siRNA delivery in the presence of HCPT.
Highlights
Previous studies demonstrated the epidermal growth factor (EGF)-targeted phagemid particles carrying Small interfering RNA (siRNA) against Akt could be expressed efficiently in the presence of hydroxycamptothecin (HCPT)
Previous studies showed that the cell-targeted phagemid particles were efficient siRNA delivery vectors in the presence of HCPT and they could efficiently deliver siRNA against Akt into targeted cells in the presence of HCPT [10]
We purified Single-stranded DNA (ssDNA) from phagemid particles to analyze the ratio of phagemids to helper phage genomes packaged in the phagemid particles
Summary
Previous studies demonstrated the EGF-targeted phagemid particles carrying siRNA against Akt could be expressed efficiently in the presence of hydroxycamptothecin (HCPT). Small interfering RNA (siRNA) molecules are capable of interrupting the translation of a specific protein by inducing post-transcriptional gene silencing It is a promising method for silencing therapeutic target genes. Since phage-based vectors do not exhibit natural tropism towards mammalian cells and can be genetically modified for specific applications, modified phage-based vectors are an attractive alternative strategy for gene delivery. They have been successfully modified to deliver genes to target cells by the effective use of targeting ligands such as growth factors, antibodies, and viral capsid proteins [1,2,3,4,5,6,7].
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