Abstract
Unlike conventional physical and chemical methods, the biogenic synthesis of gold nanoparticles (GNPs) is considered a green and non-toxic approach to produce biocompatible GNPs that can be utilized in various biomedical applications. This can be achieved by using plant-derived phytochemicals to reduce gold salt into GNPs. Several green synthesized GNPs have been shown to have antibacterial effects, which can be applied in wound dressings to prevent wound infections. Therefore, the aim of this study is to synthesize biogenic GNPs from the South African Galenia africana and Hypoxis hemerocallidea plants extracts and evaluate their antibacterial activity, using the Alamar blue assay, against bacterial strains that are known to cause wound infections. Additionally, we investigated the toxicity of the biogenic GNPs to non-cancerous human fibroblast cells (KMST-6) using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. In this paper, spherical GNPs, with particle sizes ranging from 9 to 27 nm, were synthesized and fully characterized. The GNPs from H. hemerocallidea exhibited antibacterial activity against all the tested bacterial strains, whereas GNPs produced from G. africana only exhibited antibacterial activity against Pseudomonas aeruginosa. The GNPs did not show any significant toxicity towards KMST-6 cells, which may suggest that these nanoparticles can be safely applied in wound dressings.
Highlights
The antibacterial potential of the metallic nanoparticles (NPs) have been under investigation to counter the increase of microbial resistance against the current antimicrobial agents [1]
In order to produce Gold nanoparticles (GNPs) chemically, a reducing agent is normally added to the gold salt to reduce gold atoms and allowing them to grow into GNPs
This study follows on from a previous report in which extracts from several indigenous South African plants were screened for the biosynthesis of GNPs using a quick and easy microtitre plate method [32]
Summary
The antibacterial potential of the metallic nanoparticles (NPs) have been under investigation to counter the increase of microbial resistance against the current antimicrobial agents [1]. The potential application of the NPs in wound dressings to fight infections makes these NPs extremely useful in wound care. Different metals such as gold, silver, platinum, palladium, copper, aluminum, iron, and titanium have been used to synthesize NPs [2]. GNPs are included in a variety of applications such as separation science [3], optical sensors, food industry as well as space and environmental sciences [4]. GNPs have shown potential in several biomedical applications. GNPs have been shown to destroy tumors by photothermal therapy [5]. Other biomedical applications of GNPs include gene therapy, drug delivery, DNA and RNA analysis and as antibacterial agents, etc. Other biomedical applications of GNPs include gene therapy, drug delivery, DNA and RNA analysis and as antibacterial agents, etc. [6]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
