Abstract
The specific binding of [ 3H] prostaglandin (PG) F 2α to bovine corpus luteum cell membranes was inhibited by progesterone. Progesterone Inhibition of binding was dependent on membrane protein and independent of [ 3H] PGF 2α concentrations in the medium. The lower inhibition of binding at high protein concentrations can be overcome by increasing the amounts of progesterone added. Progesterone inhibition of binding appears to be due to a decrease in the receptor number rather than a decrease in the receptor affinities. The kinetic properties (association and dissociation rates) of the remaining receptors were unchanged. The inhibition of [ 3H] PGF 2α binding was observed by preincubating the membranes with progesterone or by adding at the beginning but not during incubation. The concentrations of progesterone that inhibited binding by about 50% do occur in bovine corpora lutea of estrous cycle and pregnancy.
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