Inhibition and labeling of red beet uridine 5′diphospho‐glucose: (1,3)‐β‐glucan (callose) synthase by chemical modification with formaldehyde and uridine 5′diphospho‐pyridoxal

Abstract

The effects of the lysine‐reactive chemical modification reagents, uridine 5’ diphospho (UDP)‐pyridoxal and formaldehyde (HCHO), on the activity of membrane‐bound and solubilized UDP‐Glc: (1,3)‐β‐D‐glucan synthase (callose synthase) from red beet (Beta vulgaris L.) storage tissue were compared. Exposure to micromolar levels of UDP‐pyridoxal, or millimolar levels of HCHO in the presence of NaCNBH3, resulted in complete enzyme inactivation. Conditions for inhibition of membrane‐bound enzyme activity by the two reagents were markedly similar; divalent cations were required for inactivation, and complete protection of activity was obtained with EDTA or EGTA. The substrate, UDP‐Glc, protected membrane‐bound callose synthase against inactivation by UDP‐pyridoxal or HCHO, but protected the solubilized enzyme only against inhibition by UDP‐pyridoxal, suggesting that the lysine residue modified by both these reagents is at the enzyme active site, and that the site is more open or has a certain conformational flexibility in the solubilized enzyme.Potential UDP‐Glc‐binding polypeptides of callose synthase were identified by a two‐step labeling procedure. First, nonessential lysine residues were blocked by irreversible modification reaction with HCHO or UDP‐pyridoxal in the presence of UDP‐Glc to protect lysines at UDP‐Glc‐binding sites. In the second step, proteins were recovered, reacted with [14C]‐HCHO in the absence of UDP‐Glc, and polypeptide labeling patterns analyzed by SDS‐polyacrylamide gel electrophoresis and fluorography. This procedure reduced incorporation of label by 5‐ to 8‐fold compared to a procedure omitting the preblocking step, and with enzyme partially purified by solubilization in CHAPS followed by product entrapment, labeling was limited to a small set of polypeptides. Taken together with the results of other studies, the data suggest that one or more polypeptides migrating in the 54–57 kDa region are good candidates for the UDP‐Glc‐binding components of callose synthase.