Abstract
Aim: The purpose of this article is to prove the possibility to transfer a good amount of cells of the SVF (and ADSCs) in the infranatant portion of microfragmented adipose tissue. Method: The adipose tissue harvesting procedure is performed under local anaesthesia. The adipose tissue was harvested with a 2 mm diameter microperforated cannula with 1 mm side port holes, mounted inside the special patented guide. Both cannula and guide are included in the SEFFIHAIR™ medical device. Once the adipose tissue is harvested, it is gently washed, and it was divided in two specimens: (EMU) the tissue was emulsified with 20 passages from one syringe to another and (CTRL) the tissue didn’t undergo any emulsification. Results: The emulsification procedure liberated alive and proliferating cells and we observed that the specimens derived with a 1 mm side port hole cannula and then emulsified (EMU) showed a higher number of cells in the infranatant part compared to the one derived from the control tissue without any (1 EMU vs. 1EMU infra). Conclusion: The use of microcannulas, in combination with a mechanical digestion by an emulsification procedure and centrifuge, could ease SVF cells isolation for regenerative treatment and could also be performed in a medical facility.
Highlights
In the last years, several studies proved the efficacy of therapies based on the autologous grafting of adult mesenchymal stem cells to accelerate the healing and regenerative processes of the skin and mesenchymal tissues
In light of these results, some AA proposed the possibility of restoring the hair cycle in male and female androgenetic alopecia (AGA) using autologous fat enriched with SVF [1]
Enzymatic digestion of the adipose tissue is a gold standard procedure for progenitor cells/stem cells isolation. This procedure is time consuming; it could have an impact on cell viability and yield, and it is mandatory to perform it in equipped laboratories in order to carry out all procedures in a sterile environment
Summary
Several studies proved the efficacy of therapies based on the autologous grafting of adult mesenchymal stem cells to accelerate the healing and regenerative processes of the skin and mesenchymal tissues In light of these results, some AA proposed the possibility of restoring the hair cycle in male and female androgenetic alopecia (AGA) using autologous fat enriched with SVF [1]. A scrupulous analysis has been done on the SEFFIHAIRTM medical device we used to harvest the tissue and on its related procedure It shows to be effective in obtaining a liquid portion of alive and proliferating ADSCs. It shows to be effective in obtaining a liquid portion of alive and proliferating ADSCs These results were obtained by cell characterization which is done with an innovative technology: Celector®. Their consistency proves that this new procedure, simple and safe, can guarantee to this novel therapy an important standardization, necessary for further investigation
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