Abstract
The aim of the current experiment was to optimize the creation process of single cell protein on plant-based substrate solution with the intention to improve end-product turn out by means of adding vitamin solution. Based on the results of the fermentation processes of yeast strains, it was concluded that the vitamin-supplementation produced its greatest effect on the dry matter production, primarily on the K. marxianus DSM 4908 strain, while it was less beneficent when it comes to the figures of wet cell mass. In addition, it can be assumed that vitamin supplementation increased the maximum specific rate of growth (μmax) and decreased the generation time (tg) significantly. In the case of the K. marxianus yeast strain on corn steep liquor treated with vitamin-supplementation, the highest (μmax) and the lowest (tg) data were observed [(0.226 h-1) and (4.4 h), respectively]. Based on the results it was found that K. marxianus DSM 4908 is expedient to be applied on corn steep liquor medium in order to determine its suitability to produce additive for feeding.
Highlights
As a result of the expansion of fermentation biotechnology, the growth of the significance of creating single cell protein supplementation additives may appear on the market
Wet cell mass and yeast cell count values were determined by using samples gained from the optimisation fermentation processes
The experiment was repeated by adding vitamin solution, but not changing the optimised parameters
Summary
As a result of the expansion of fermentation biotechnology, the growth of the significance of creating single cell protein supplementation additives may appear on the market. These protein supplementation additives feature perfect quality indicators and can be exploited in the forage industry. Saccharomyces cerevisiae and Kluyveromyces marxianus strain of yeast species are applied in increasing numbers to produce these products. In these fermentation processes, the most commonly used substrate of plant origin in molasses, corn steep liquor is a popular yeast medium. It was an objective to improve yeast reproduction, as well as production indicators by adding vitamin solution
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