Abstract
An experiment was conducted to develop a regeneration protocol from seedling explants of higher yield Psidium guajavacv. safeda. Initially surface sterilized guava seeds were germinated inside the conical flasks. Explants taken from 49-day old seedlings were cultured on MS-medium modified with different concentration of plant growth regulators. Maximum explants showing shoot response (48%) was observed on MS-medium supplemented with 6-benzyladanin (BA) 1.0 mgl-1combined with 0.5 mgl-1ziatin. On the same media after 38-days of culturing, maximum number of 3.6 shoots per explant, 2.6 cm long was also recorded. On MS containing combination of BA (2.0 mgl-1) and zeatin (1.0 mgl-1), 37% explants responded which produced 2.3 shoot per explant after 42 days of culturing. Whereas on MS containing glutamin 250 mgl-1, 25% explants responded and gave 2.2 shoots per explants after 46-days. However, when the concentration of glutamin alone was increased from 250 to 500 mgl-1 the shoot formation potential of explant decreased to 10%. Addition of BA, glutamin and zeatin alone to MS medium was less effective. However, combination of BA with zeatin was effective in shooting response, number of shoot/explants and shoot length. Maximum number of explants (76.6%) responded to rooting with 1.5 mgl-1 IAA in combination with 1.0 mgl-1 NAA. On average, maximum of (2.5) roots were also recorded per explants in 42 days. In vitro plantlets were acclimatized and shifted to soil successfully. Keywords: Psidium guajava L; Micropropagation; Zeatin; Glutamin and auxins http://dx.doi.org/10.19045/bspab.2017.60014
Highlights
Introduction from Mexico toPeru [2]
Root organogenesis The Micro shoots produced from seedling explants on MS media supplemented with different cytokinin were excised and shifted to rooting medium containing different concentrations and combinations of Indol-3butyric acid (IBA), Indole-3-acetic acid (IAA) and Naphthalene acetic acid (NAA)
25% explants responded to shoot regeneration when glutamin 250 mgl-1 was added to MS medium
Summary
Introduction from Mexico toPeru [2]. Guava is grownGuava (Psidium guajava L.) is one of the throughout the tropical and subtropical most common fruit crops of Pakistan which regions of the world but the average yield, ranks third after citrus and mango in area is much less than its potential, and production wise [1]. Maximum number of explants (76.6%) responded to rooting with 1.5 mgl-1 IAA in combination with 1.0 mgl-1 NAA. Root organogenesis The Micro shoots produced from seedling explants on MS media supplemented with different cytokinin were excised and shifted to rooting medium containing different concentrations and combinations of Indol-3butyric acid (IBA), Indole-3-acetic acid (IAA) and Naphthalene acetic acid (NAA).
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