Abstract
Citrus tristeza virus (CTV) is transmitted in a semipersistent manner by several aphid species, among which Toxoptera citricida is most efficient. After 24 h acquisition access period, eight CTV isolates were detected in single T. citricida with frequency of 63.3-91.1% and 71.1-91.1% by nested RT-PCR and real-time RTPCR, respectively. More copies of CTV-targets were detected in single viruliferous T. citricida individuals that acquired high transmissibility CTV isolates. Analysis by single strand conformation polymorphism (SSCP) of viruliferous T. citricida individuals that had acquired CTV isolates ST9, GS14, CT11, HH12, and of CTV-infected receptor plants showed that most of the CTV-infected receptor plants and individual of viruliferous aphids carried one CTV haplotype, and that CTV in the receptor plants showed less haplotype diversity than that in aphids.
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