Abstract
The molecular mechanisms that control the mycotoxin-mediated effects in porcine endometrial cells are far from being completely understood. Recent results show that they could inhibit cell proliferation. Therefore, the present study investigated the effects of the mycotoxins α-zearalenol (α-ZOL) and β-zearalenol (β-ZOL) on a cellular level. Mainly, the abundance and phosphorylation state (activity) of the cell cycle-dependent kinases MAPK and Akt (PKB) and their potential targets eIF4E (eukaryotic initiation factor 4E) and 4E-BP1 (4E binding protein, eIF4E repressor protein) were investigated. The results show that α-ZOL has apparently only a slight influence on the phosphorylation state of MAP kinases, Akt and on eIF4E and 4E-BP1. In contrast, their phosphorylation was strongly reduced in β-ZOL-treated cells in a concentration-dependent manner. Therefore, our results indicate that β-ZOL potentially not only influences transcription but also effects gene expression on translational level. The effect of α- and β-ZOL on endometrial cell proliferation and their toxicology are discussed.
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