Abstract
The growth of Spirulina platensis is dependent on culture conditions. This study has established adequate conditions for the quality and quantity production of S. platensis. The effect of sulphate salts nutrition on growth performance and biochemical status of S. platensis was assessed in vitro. Prior to culture, the Paracas strain of S. platensis from SAGRIC pond was analysed in different magnesium sulphate (MgSO4; 0.08, 0.16, 0.32, 0.64 and 1.28 g/L), potassium sulphate (K2SO4; 0.08, 0.16, 0.32, 0.64 and 1.28 g/L) and MgSO4/K2SO4 (0.16/0.00, 0.08/0.08, 0.04/0.12, 0.02/0.14 and 0.01/0.15 g/L) concentrations. Culture media pH, total dissolved solids (TDS) and conductivity rate were monitored. Microscopic analysis revealed sulphate salt concentrations influenced the number of whorls and filaments of S. platensis. K2SO4 (1.28 g/L) produced the highest number of whorls and filaments. Moreover, pH level fluctuated by sulphate treatments. K2SO4 (1.28 g/L) had a pH level of 8.77±0.01 (day 5 of culture incubation). TDS and conductivity rate, protein and cysteine contents increased with culture age and K2SO4 concentration in a culture medium. Conversely, negative correlations between protein and cysteine contents were observed, and sugar content decreased. Sulphate salt type and concentrations affected polyphenol oxidase (PPO) and peroxidase (POD) activities. MgSO4/K2SO4 (0.02/0.14 g/L) displayed the best PPO and POD activities. Both enzymes appeared to be negatively correlated to the decreasing sugar content. These results indicate growth performances and biochemical status of S. platensis are significantly improved with the adequate supplementation of sulphate salts (MgSO4 and K2SO4) in culture media.
Highlights
Spirulina platensis is a multicellular, filamentous and microscopic photosynthetic cyanobacterium commonly found in the brackish lakes of Central Africa and Mexico
It was noticed that S. platensis was grown at 27.7±0.03 °C
The present study revealed pH fluctuation along the culture process
Summary
Spirulina platensis is a multicellular, filamentous and microscopic photosynthetic cyanobacterium commonly found in the brackish lakes of Central Africa and Mexico. S. platensis has been consumed for centuries by the Aztecs and bordering populations on Lake Chad (Shigekatsu et al, 2019) This microalgae is characterized by a high content of protein (including enzymes such as polyphenol oxidase and peroxidase) and high amounts of essential fatty acids, essential amino acids, minerals, vitamins (especially B12), polysaccharides and antioxidant pigments (chlorophyll, carotenoids, phycobiliprotein, phycocyanin and carotenoids) (Budiyono et al, 2014; Ben Amor et al, 2017; Jung et al, 2019; Fatemeh & Choopani, 2020). It is possible to improve the growth performance, biochemical status and antioxidant activity of S. platensis while acting on composition of culture medium in order to fulfil pharmaceutical and nutritional requirements
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