Abstract
A study was conducted at the tissue culture lab. College of Agriculture/ University of Baghdad and Drug Research Center/ Health Minstry during 2006 - 2007. Results indicated that MS medium supplemented with 30 g/l of sucrose gave the highest values (630.3, 61.6) mg of both fresh and dry weight of callus respectively. However, values of both parameters were reduced with increasing sucrose concentrations up to 60, 90, 120 g/l (478.2, 346.5, 217.6) mg respectively. MS medium supplemented with 30 mg/l of tyrosine gave the highest values of callus fresh and dry weight (429.7, 42.6) mg respectively. MS medium supplemented with 30 g/l of sucrose and 30 mg/L tyrosine gave the highest values (688.0, 67.5) mg of both fresh and dry weight callus. MS medium supplemented with 30 mg/l of tyrosine and 90 g/l of sucrose gave the highest values (2.9, 2.8) mg/g of morphine and codeine from fresh callus weight respectively while the lowest values of morphine and codeine (0.5K 0.2) mg/g from fresh callus weight respectively of control treatment .
Highlights
S (688.0, 67.5) mg of both fresh and dry weight callus
ثم قلت الاستجابة بزيادة تركيز التايروسين الى 40ملغم/لتر اذ بلغ ) (2.4 ، 2.7ملغم/غم مورفين وكودائين على التوالي شكل) (9ثم قلت الاستجابة لانتاج القلويدات في الوسط الغذائي المجھز بتركيز 120غم /لتر سكروز وتراكيز مختلفة من التايروسين ،اذ بلغ ) (0.8 ، 1.1ملغم /غم مورفين وكودائين على التوالي في معاملة المحايد كما في شكل ) . (10وبلغت اعلى قيمة في الوسط الغذائي نفسه عند التركيز 30ملغم /لتر من التايروسين اذ بلغ ) (1.9 ، 2.3ملغم /غم مورفين وكودائين على التوالي كما في شكل ) ، (11وبعدھا قلت الاستجابة بزيادة تركيز التايروسين الى 40ملغم /لتر اذ بلغ ) (1.7 ، 2.2مورفين وكودائين على التوالي كما موضح شكل )
شكل) :(9كمية المورفين والكودائين )ملغم/غم( في وسط مجھز بـ 90غم/لتر سكروز و 40ملغم/لتر تايروسين
Summary
S (688.0, 67.5) mg of both fresh and dry weight callus. MS medium supplemented with 30 mg/l of tyrosine and 90 g/l of sucrose gave the highest values معدل وزن الكالس الجاف بزيادة تراكيز السكروز الى ) (120 ، 90 ، 60غم/لتر فبلغ )، 34.3 ، 46.8 تأثير تراكيز السكروز والتايروسين في محتوى الكالس الطري من المورفين والكودائين )ملغم/غم(
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