Abstract
Objective To investigate the influence of storage temperature and duration of specimens on detection of CD64. Methods 10 samples of venous blood anticoagulated with EDTA-K2 were collected.Each sample was placed in two tubes and stored at room temperature and 4℃ separately. The neutrophil CD64 levels in the samples were detected by flow cytometry 30min, 6h, 12h, and 24h after collection. Results The expression level of CD64 in neutrophils increased with time. CD64 was ( 1540.1 ± 1141.0 )PE\cell at 30 min and was ( 1867.8 + 1186.0 ) PE\cell at room temperature and ( 1730.6 ± 1221.5 ) PE\cell at 4℃ after 24-hour storage, showing a significant difference in the expression level of CD64 between two different temperatures and two sorts of storage duration ( P ＜ 0.05 for both comparisons ). Conclusions Blood samples should be stored at 4℃ and detected within 12h after collection to ensure accuracy of the results. Key words: CD64; Temperature; Duration
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