Abstract
Cells migrate from the limbus to the corneal epithelium following a centripetal pathway. Corneal epithelial cells tend to orientate in spiral or vortex patterns. However, when cultured in-vitro, limbal derived corneal epithelia do not tend to align or migrate in a spiral pattern. Here, we used soft lithography to create silk fibroin substrates with spiral topographies that direct the human limbal-derived immortalized corneal epithelial cells (hTCEpi) to form a spiral orientation. The impact of this topography on the cells was then characterized. The spiral patterns affected cytoskeletal organization, cell spreading, and nuclei shapes. Spiral width and numbers had a significant impact on proliferation of cells, their focal adhesion, their chromatin condensation, and number of actin filaments. Immunocytochemical staining showed that the spiral pattern enhanced the expression of markers associated with limbal stem cells. The current work illustrates micro spiral patterns can serve to control the nature of limbal derived epithelial cells by providing relevant biophysical cues.
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