Abstract
We have examined the influence of psychoactive [Δ 9-tetrahydrocannabinol (Δ 9-THC), Δ 8-THC and 11-OH-Δ 9-THC] and nonpsychoactive (cannabinol) cunnabinoids on proliferation of human cells (HeLa S 3 cells grown in suspension culture) and on biochemical events associated with the proliferative process. The four cannabinoids studied brought about a dose-dependent inhibition in cell growth. Pulse-labeling studies indicated a cannabinoid-induced decrease in incorporation of [ 3H]thymidine, [ 3H]uridine and [ 3H]leucine into DNA, RNA and protein, respectively, in intact cells: in most cases a comparable decrease in the acid-soluble intracellular precursor pools was observed. Results from in vitro nuclear and chromatin transcription experiments did not suggest a drug-induced change in RNA synthesis. It appears, therefore, that quantitative alterations in the synthesis and/or turnover of nucleic acids and proteins do not result from cannabinoid treatment of HeLa cells. Our data, however, do not preclude the possibility that cannabinoids affect the synthesis of specific molecules.
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