Abstract
Phosphoglycerate mutase (EC 2.7.5.3) of Leuconostoc dextranicum AHU 1078 adsorbed onto blue dextran-Sepharose, was easily eluted with a low concentration of pyrophosphate. Pyrophosphate inhibited the mutase competitively with respect to 3-phosphoglycerate, but noncompetitive^ with respect to 2,3-bisphosphoglycerate. Further inhibition studies suggested that two molecules of pyrophosphate bound per active site of the phosphoglycerate mutase. The inactivation of the Leuconostoc mutase by pyridoxal phosphate was prevented by the addition of pyrophosphate. Spectral measurements revealed that the mutase-Cibacron Blue complex in solution was dissociated by the addition of pyrophosphate.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
