Abstract
A rapid capillary zone electrophoresis (CZE) method was developed using a 3-(cyclohexylamino)-1-propanesulfonic acid (CAPS) buffer for the compound-class separation of nine polycyclic aromatic hydrocarbon (PAH) metabolites including tetrols, benzo[ a]pyrene diols, and hydroxyl aromatics. In addition, the effects of two organic solvents (methanol and acetonitrile) on the electroosmotic flow and electrophoretic mobilities of the analytes were studied. Separation of nine PAH metabolites was ultimately achieved within 24 min with a 100 m M CAPS buffer with 40% (v/v) MeOH at an apparent pH of 10.4. In addition, it was possible to partially resolve the parent PAH compounds from the metabolites with the previous buffer system. The simple CZE methods developed would be useful for the rapid separation and characterization of several important biomarkers.
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