Influence of litter size on antepartum serum relaxin and progesterone immunoactivity levels and on birth in the rat.

Abstract

In the rat, the antepartum elevation of serum relaxin levels consists of two phases separated by a 24-h interval. The second phase, which occurs between 36 and 24 h before birth, is temporally closely associated with functional luteolysis. Relaxin levels then decline throughout the last approximately 24 h of pregnancy. We have postulated that the two phases in the antepartum elevation of serum relaxin levels may be indicative of an increasingly effective endogenous circadian luteolytic process. There is limited evidence that both luteolysis and birth are delayed in rats with small litters. The present study investigated in detail the relationship between litter size and the timing of both functional luteolysis and birth in rats. The number of conceptuses (C) in Sprague-Dawley-derived rats was surgically adjusted on day 8 of pregnancy (day 8) so that rats bore one, two, three, five, or a full complement (FC) of eight or more C. Rats were maintained under a photoperiod regimen of 14 h of light and 10 h of darkness (lights on from 2100-1100 h) beginning on day 8 and observed for birth at 10-min intervals from 2100 h on day 22. Serum levels of both relaxin and progesterone were determined in blood samples obtained at 4-h intervals from 2400 h on day 19 until birth. Ninety-five percent of the rats that had five or more C gave birth during the light phase on day 23, which was designated the normal birth interval. However, only 20% of the rats with three C or less, gave birth during the normal birth interval, and 47% gave birth about 24 h later during the light phase on day 24, which was designated the late birth interval. The 24-h delay in birth of rats with small litters which delivered during the late birth interval appears to be attributable to a delay in functional luteolysis; the antepartum decline in serum relaxin and progesterone levels occurred about 24 h later in these rats than in rats that delivered during the normal birth interval. It is concluded that the C may be associated with the luteolytic process and thereby influence the time of birth in rats. Additionally, the results of this study are consistent with our hypothesis that there is an endogenous circadian luteolytic process in rats during the antepartum period.