Abstract
BackgroundChromosomal rearrangements such as duplications and deletions are key factors in evolutionary processes because they promote genomic plasticity. Although the genetic variations in the Saccharomyces cerevisiae species have been well documented, there is little known to date about the impact of the genetic background on the appearance of rearrangements.ResultsUsing the same genetic screening, the type of rearrangements and the mutation rates observed in the S288c S. cerevisiae strain were compared to previous findings obtained in the FL100 background. Transposon-associated rearrangements, a major chromosomal rearrangement event selected in FL100, were not detected in S288c. The mechanisms involved in the occurrence of deletions and duplications in the S288c strain were also tackled, using strains deleted for genes implicated in homologous recombination (HR) or non-homologous end joining (NHEJ). Our results indicate that an Yku80p-independent NHEJ pathway is involved in the occurrence of these rearrangements in the S288c background.ConclusionThe comparison of two different S. cerevisiae strains, FL100 and S288c, allowed us to conclude that intra-species genomic variations have an important impact on the occurrence of chromosomal rearrangement and that this variability can partly be explained by differences in Ty1 retrotransposon activity.
Highlights
Chromosomal rearrangements such as duplications and deletions are key factors in evolutionary processes because they promote genomic plasticity
The genetic background affects the occurrence of chromosomal rearrangements In previous studies, selections were carried out in a FL100 ura215,30,70 context
Among the chromosomal rearrangements leading to ATCase reactivation in FL100, Ty1 insertions usually account for 66%, deletions for 17% and duplications for 17% (Figure 2A) [6,7]
Summary
Chromosomal rearrangements such as duplications and deletions are key factors in evolutionary processes because they promote genomic plasticity. DNA double strand breaks occur spontaneously or as a result of DNA damaging agents such as ionizing radiations or chemical reagents If this damage is not properly repaired, it can lead to the occurrence of chromosomal rearrangements such as duplications, deletions and translocations, which can affect cell growth and survival. These rearrangements are key events in genome reshaping and evolution processes and many of the genomes sequenced to date show traces of these rearrangements [1,2]. BMC Genomics 2009, 10:99 http://www.biomedcentral.com/1471-2164/10/99 the integrity of the genome and prevent the deleterious effects of chromosomal rearrangements These mechanisms can be classified in two distinct pathways: the homologous recombination (HR) pathway and the nonhomologous end-joining (NHEJ) pathway. HR requires long homologous sequences for DSB repair whereas little or no homology is necessary for the NHEJ pathway
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