Influence of frequency of feeding containing fitase on efficiency of food utilization and growth of common carp (Cyprinus carpio) fingerlings

The experiment was conducted for 112 days in 20 Aquariums with dimension of 80×30×30 cm and a capacity of 70 liters. A hundred and eighty (180) common carp fingerlings (Cyprinus carpio L.) were distributed randomly on seven treatments with three replicates per treatment except control (2 replicates). Each replicate had 9 fingerlings/ aquarium with an average body weight of 10.37 ± 0.14 g/ fish. Fish were fed seven experimental diets which were mixed locally with a protein content of 31.79- 32.24% and a metabolizable energy of 1428.194-1437.919 kcal./kg feed. Probiotic and prebiotic were added as 5, 10 and 15 gm/ Kg feed to the experimental diets except for the control diet. The results indicated no significant differences (P>0.05)among fish fed the experimental diets in final body weight, weight gain, daily weight gain, relative growth rate, feed conversion ratio, feed efficiency ratio, protein efficiency ratio and the amount of protein consumed by fish throughout the study period. However the specific growth rates of the experimental diets were significantly different (P<0.05) compared to the control diet.

This study was undertaken to evaluate the effects of apple cider vinegar on growth performance and non-specific immune parameters of skin mucus (alkaline phosphatase, lysozyme and total protein) in common carp fingerlings. For this purpose, a total of 240 fish were stocked in twelve tanks for four treatment with three replications and fed by diets supplemented with 0, 1, 2 and 4 % apple cider vinegar for 60 days. The result showed that there was no significant difference in body weight increase (BWI), feed conversion ratio (FCR), specific growth rate (SGR) and condition factor (CF) among the treatments ( P >0.05). The results revealed that there is no significant difference among skin mucus alkaline phosphatase of fish in all treatments. Although skin mucus lysozyme activity and total protein significantly increased by apple cider vinegar in comparison with the control group. The highest and lowest skin mucus lysozyme activity and total protein in common carp fingerlings were observed at 2% apple cider vinegar and control group, respectively. Therefore, apple cider vinegar improves skin mucus lysozyme activity and total protein and can be a good candidate for antibiotic replacement in common carp fingerlings.

IntroductionBiofloc technology (BFT) offers a sustainable approach to aquaculture by enhancing water quality and nutrient utilization. This study investigated the effects of varying carbon-to-nitrogen ratios, achieved through different combinations of jaggery and corn flour, rice flour, or rice bran, on the growth performance, body composition, histological health, and immune response of common carp (Cyprinus carpio) fingerlings in a BFT system.MethodsA 90-day experiment was conducted using common carp fingerlings, where different groups were fed diets supplemented with jaggery in combination with either corn flour, rice flour, or rice bran, creating varying C:N ratios. Water quality parameters, Biofloc volume, growth performance (final weight, weight gain, specific growth rate), body composition (crude protein, crude lipid), histological analysis of liver and intestine, Biofloc morphology, and immune-related gene expression were assessed.ResultsThe addition of carbon sources, particularly jaggery and rice flour, significantly improved water quality by reducing total ammonia nitrogen, nitrite, and nitrate levels, and increasing Biofloc volume. The jaggery and rice flour group exhibited the highest final weight, weight gain, specific growth rate, and levels of crude protein and crude lipid. Histological analysis showed improved hepatic and intestinal health in this group. Biofloc morphology varied, with rod shapes observed in the jaggery and rice flour group and spherical peanut shapes in the jaggery and corn flour group. The jaggery and rice flour group also demonstrated elevated expression of immune-related genes.DiscussionThe combination of jaggery and rice flour provided an optimal C:N ratio, leading to enhanced water quality, improved growth performance, better body composition, healthier histological structure, and a strengthened immune response in common carp fingerlings. The unique Biofloc morphology and increased immune gene expression observed in this group suggest a synergistic effect of jaggery and rice flour in promoting a robust and efficient BFT system for common carp aquaculture.

Effects of different carbon to nitrogen ratios in the biofloc system with sugarcane molasses as organic carbon source on water quality, growth performance, and body composition of common carp (Cyprinus carpio L.) fingerlings were investigated in a 90-day trial. A total of 450 fish (14.17 ± 0.36 g) were fed twice daily with commercial diet at 5% of their body weight under a 12:12 light:dark regime and cultured in 1 control (without extra carbon source) and 4 experimental treatments (C/N: 11:1, 15:1, 19:1, and 23:1) with 3 replicates at a density of 1.06 kg m−3 per tank. Based on the results, the water electrical conductivity (EC) and total dissolved solids (TDS) (measured daily), flocs volume (FV), and total suspended solids (TSS) (measured once a week) were increased from C/N 11:1 to C/N 23:1. The variation patterns of the average levels of total ammonia nitrogen, nitrite, and nitrate (measured once a week) were similar and showed a decreasing trend with an increase in C/N ratio. Total heterotrophic bacteria was also positively affected by increasing C/N to 23:1. The highest fish final weight (59.36 ± 0.24 g), weight gain (45.21 ± 0.47 g), and protein efficiency ratio (PER, 1.77 ± 0.04) were recorded in C/N 19:1. The lowest (1.46 ± 0.03) and highest (1.51 ± 0.05) feed conversion ratio (FCR) and the highest (1.60 ± 0.03%/day) and lowest (1.49 ± 0.01%/day) specific growth rate (SGR) were observed in C/N 19:1 and the control group, respectively (P < 0.05). By increasing the C/N to 23:1, FCR increased and the feed intake, final weight, weight gain, PER, and SGR decreased. The highest crude protein content (77.31 ± 0.43 %DW) and the lowest crude lipid content (12.13 ± 0.32 %DW) of the fish carcass were observed in C/N 19:1 while these values for flocs were not significantly affected by C/N ratio. Overall, the biofloc system with C/N 19:1 improved the water quality and growth performance of common carp while not negatively affecting the carcass proximate analysis.

Enrichment of common carp (Cyprinus carpio) diet with medlar (Mespilus germanica) leaf extract: Effects on skin mucosal immunity and growth performance

Rosemary leaf powder improved growth performance, immune and antioxidant parameters, and crowding stress responses in common carp (Cyprinus carpio) fingerlings

Enrichment of common carp (Cyprinus carpio) fingerlings diet with Psidium guajava: The effects on cutaneous mucosal and serum immune parameters and immune related genes expression

Sunflower meal based diet supplemented with selenium nanoparticles (Se NPs) atlevels of 0, 1, 2, 3, 4 and 5 mg kg-1 were fed common carp (Cyprinus carpio Linnaeus 1758) fingerlings in a seventy days feeding trial, to examine the effects on growth, nutrient digestibility and heamatology. Triplicate tanks were used and each tank contained 15 fingerlings that were subjected to experimental feeding at 4% of their live wet weight. A non-digestible marker, chromic oxide was added in the feed at 1% level. Growth performance in terms of weight gain %, feed conversion ratio (FCR) and specific growth rate (SGR) as well as nutrient digestibility (crude protein, crude fat, gross energy) were estimated following standard methods/standard formulae. Maximum weight gain% (210%), SGR (1.26) and best FCR (1.26) as well as highest nutrient digestibility (crude protein 71%, crude fat 72% and gross energy 70%) were observed in fish fed test diet III supplemented with 2 mg kg-1 Se NPs These results showed that, Se nanoparticles supplementation at the rate of 2 mg kg-1 level is beneficial for improvement of growth in C. carpio fingerlings.

The present study investigates the effects of varying levels of dietary fermented Aspergillus oryzae (0 g Kg-1 as control group, 10, 20 and 30 g Kg-1) on performance and haemato-immunological indices of common carp (Cyprinus carpio) fingerlings. Common carp fingerlings (n=240, w=4.56±0.17 g) were supplied, randomly stocked in 12 aquaria and fed with experimental diets for 7 weeks. The results revealed no significant differences between performance parameters of fermented A. oryzae fed test and control diets (p˃0.05). Also, evaluation of haematological parameters (RBC, Hct, Hb) and white blood cell (WBC) counts showed no notable changes between experimental groups (p˃0.05). However, respiratory burst activity was meaningfully higher in fish fed fermented A. oryzae compared to control group (p<0.05). The present results showed that possible effects of administration of fermented A. oryzae on immune responses in carp fingerlings.

The purpose of this study was to determine the effect of adding phytase enzyme in the feed on digestibility of feed, efficiency of feed utilization, relative growth rate and survival rate of Common carp (Cyprinus carpio). Fish samples in this research were Common carp with an average - weight of 3.34 ± 0,16 g/fish. The treatments were adding the phytase enzyme in the feed with the different level of doses. Those were A (0 U kg-1 feed), B (500 U kg-1 feed), C (1.000 U kg-1 feed g) and D (1.500 U kg-1 feed). Observation was conducted on digestibility of protein (ADCP), digestibility of phosphor (ADCF), efficiency of feed utilization (EFU), relative growth rate (RGR), protein efficiency ratio (PER), feed conversion ratio (FCR), survival rate (SR) and water quality parameters. The results show that the addition of phytase enzyme significantly (P<0.01) affected on ADCP, ADCF, EFU, RGR, FCR, and PER, on the other hand it insignificantly (P>0.05) affected on SR of common carp. Based on results, it was concluded that optimum doses of phytase enzyme feed in terms of digestibility of feed, efficiency utilization of Feed and growth rate of Common carp ranges from 943 to 1100 U kg-1 feed

Dietary oregano essential oil improved the growth performance via enhancing the intestinal morphometry and hepato-renal functions of common carp (Cyprinus carpio L.) fingerlings

O presente estudo teve por objetivo determinar a exigência de lisina para alevinos de carpa comum (Cyprinus carpio). Foram distribuí­­dos inteiramente ao acaso 280 alevinos de carpa comum com peso e comprimento inicial médio de 0,62 ± 0,05 g e 3,38 ± 0,11 cm respectivamente em 28 tanques-rede quadrados com volume útil de 150 litros, dispostos no interior de um tanque circular de alvenaria com capacidade para 25 m3 de água (sete tratamentos e quatro repetições). Os peixes foram alimentados até a saciedade aparente quatro vezes ao dia com dietas isproteicas e isoenergéticas suplementadas com lisina nas concentrações de 1,58; 1,81; 2,05; 2,29; 2,53; 2,76 e 3,0% da dieta por 57 dias. Ao final do estudo foi avaliado o desempenho produtivo, sobrevivência e composição centesimal da carcaça dos peixes. Os dados obtidos foram submetidos a análise de regressão (p&lt;0,05) e ao teste múltiplo de média Tukey. A análise do platô de resposta linear (LRP) foi aplicado adicionalmente. A suplementação de lisina melhorou (p&lt;0,05) o peso final médio, ganho de peso médio e taxa de crescimento especí­­fico e, a partir de 2,29% de suplementação de lisina na dieta, prejudicou a eficiência alimentar dos peixes. A exigência de lisina para alevinos de carpa comum é de 1,89% da dieta ou 4,73% da proteí­­na bruta para obtenção do melhor crescimento.

Influence of salinity on food consumption, growth and energy conversion efficiency of common carp ( Cyprinus carpio) fingerlings

This study was conducted to determine the effect of direct transportation of the common carp (Cyprinus carpio) fingerlings to salt solution of 4, 6, 8 and 12 g / L concentrations from a control solution of 2.2 g / L on growth and nutrition. The high mortality 100 % occurs during the first 24 hours at 8 and 12 g / L solution respectively. No fish mortality occurred at the treatment of 4 and 6 g / L during the same period. Statistical analysis of final weight, weight increase, daily growth rate, specific growth rate and relative growth rate showed no significant differences (P&gt;0.05) between treatment 4 g / L and the control, but the treatment of 6 g / L exhibited significant different (P&lt;0.05) with the control. The values of food intake for the body weight reached 3.59, 3.01 and 2.39 % for the treatments control, 4 g / L and 6 g / L respectively, with significant difference (P&lt;0.05).

Bacterial probiotics (Bacillus subtilis) and dietary supplements improve the growth and biochemical performance of Common carp (Cyprinus carpio) fingerlings. Fish were nourished for 60 days with a control diet and experimental diets containing B. subtilis 106 to 109 CFU/ ml-l respectively. Dietary supplementation of B. subtilis feeding trial was conducted for 60 days to improve water quality and growth parameters were observed through weight gain, Specific growth rates, and Percent survival of C. carpio fingerlings all through the test period.