Influence of dietary arginine and glycine content on thyroid function and growth of juvenile rainbow trout, Oncorhynchus mykiss (Walbaum)

Abstract

Juvenile rainbow trout, held at 12°C on a 12 h light :12 h dark photocycle, were fed a constant ration (1 % of body wt day −1) of isonitrogenous and isoenergetic diets that varied in either arginine content (3.6-56.1 g kg−1 dry matter; experiment 1), or glycine (3.3–118 g kg−1 dry matter) and alanine (5.0-42.3 g kg−1 dry matter) content (experiment 2). In experiment 1, the lowest dietary level of arginine depressed growth, feed efficiency, plasma l-thyroxine (T4) and 3,5,3′-triiodo-l-thyronine (T3) levels and hepatic T4 5’monodeiodinase (5'D) activity responsible for T4-to-T3 conversion. Over the dietary range of 7.1–56.1 g arginine kg−1, there was no change in 5'D activity, despite an arginine stimulation of growth. The optimum level of arginine for growth was within the range of 14.1–28.1 g kg−1 of the diet or 32–63 g kg−1 of dietary protein. In experiment 2, an increase in dietary glycine level, at the expense of glutamic acid, increased 5'D activity without attendant elevation of the plasma T3 level. The latter finding suggests that glycine also induced a compensatory increase in T3 degradation rate. This may explain why the glycine-induced increase in 5'D activity was unaccompanied by any changes in growth indices. Alteration of dietary alanine content did not affect growth or thyroid function. We conclude that of the various dietary amino acids tested, only glycine led to a progressive stimulation of hepatic T4 5'D activity. However, because glycine likely enhanced T3 degradation, no increases in plasma T3 or growth indices were found. Glycine may serve as an advance signal that activates thyroid function immediately preceding or coincident with energy and nutrient (especially protein) intake. This, in turn, may improve the efficiency of nutrient absorption and/or post-absorptive anabolic events.