Abstract
Abstract The goal of this work was to use glycerol produced from biodiesel synthesis to grow Candida sp. LEB-M3 and select significant variables of culture conditions on lipid production. This yeast was isolated from a Brazilian biome which showed capacity to accumulate up to 55% lipids (w/w) and convert about 43% of glycerol into lipids. Production and lipid profile at different growth temperatures were studied. The experimental design showed that glycerol, FeCl 3 ·6H 2 O, yeast hydrolyzate and temperature were significant on the lipid production. Cultivation at 23 °C promoted the highest concentration of lipids about 9.9 g/L. However, the lipid profiles for the different growth temperatures were similar, with high concentrations of linoleic acid (C18:2) (∼45–55%) and smaller amount of gamma linolenic acid (C18:3) (∼2–5%), both essential fatty acids, leading to the conclusion that lipid produced by Candida sp. LEB-M3 has potential to be used both as a feedstock for biodiesel production or as a source for essential fatty acids.
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