Influence of Application of Various Doses of Lime Immeliorant into the Soil on QTL Mapping in Spring Bread Wheat (<i>Triticum aestivum</i> L.)

Abstract

In this work, quantitative trait loci (QTL) were mapped for the first time in spring bread wheat (Triticum aestivum L.) when various doses of a lime ameliorant, raw-milled dolomite, were introduced into the soil in order to reveal and identify genetic determinants that determine the ecological genetic interaction “genotype–environment” in recombinant inbred lines of this crop. As a result of the research, 29 economically valuable traits were evaluated and a total of 150 QTLs were mapped. The significance of the relationship between the identified QTLs and polymorphism for each studied trait was established based on the threshold values of the likelihood ratio of the logarithm of odds (LOD-score). It was found that when half and full doses of the ameliorant were applied to the soil, the QTLs that determine the manifestation of 14 studied traits changed their location on the linkage groups depending on the applied dose of raw ground dolomite, and the QTL of 15 traits remained stable and did not change the localization on the chromosomes established in the experiments. Conducted correlation and one-way analysis of variance made it possible to establish the nature of the conjugation of the relationship between the traits and the dose of the introduced lime ameliorant. When performing mathematical calculations, the maximum likelihood criterion and statistical criteria for assessing the significance of the results were used. The results obtained are of interest for the subsequent study of the ecological and genetic mechanisms of the realization of the studied traits and the control of growth, development and productivity in spring soft wheat, and the establishment of trigger mechanisms for the action of the ameliorant on the physiological state of plants.