Abstract
The effect of 5-fluorouracil, ionizing radiation and the microtubular inhibitor nocodazole on growth and directional migration of virally transformed malignant C3H mouse fibroblastic cells (MO4) is compared with their effect on the invasiveness of these cells in vitro. The increase in diameter of individual aggregates of MO4 cells in shaker culture is used as an index of growth. The mean diameter of the circular area covered by MO4 cells migrating from an aggregate explanted on glass is used as an index of directional migration. Invasion is studied using confrontations of aggregates of MO4 cells with precultured fragments of embryonic chick heart in shaker culture. Ionizing radiation (5000 R) and 5-fluorouracil (1 μg/ml) permit directional migration and invasion for at least 14 days after the onset of treatment, although they inhibit growth. The microtubule-inhibitor nocodazole (1 μg/ml) inhibits growth and prevents both directional migration and invasion. We conclude that various anti-cancer agents, at doses that inhibit growth, have different effects on invasion. We suggest that the assay of directional migration is used for the screening of potential anti-invasive agents.
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