Influence of absorption spectrum with 650 nm low-level laser irradiation on in-vitro hyperlipidemia blood and erythrocyte

Abstract

Objective To study the effect on absorption spectrum of low-level laser irradiation on hyperlipidemia blood and erythrocyte in vitro. Methods A total of 30 hyperlipidemia blood samples were chosen as the experiment group, and 30 normal blood samples were chosen as the control group. The absorption spectrum of whole blood and erythrocyte in the two groups was measured by multi-wavelength microplate reader and compared. After hyperlipidemia and normal blood samples were irradiated by 650 nm low-level laser, the absorbance of whole blood and erythrocyte was measured, and the results were compared with those of hyperlipidemia and normal blood samples before irradiation. Results The absorption peaks (416, 544, 578 nm) of whole blood and erythrocyte in the experiment group were higher than those in the control group, which was considered as a diagnostic factor of hyperlipidemia. After low-level laser irradiation, the absorption peaks of erythrocyte at 416, 544, and 578 nm in the experiment group decreased with significant differences (P<0.05), while the absorption peaks of whole blood at 416, 544, and 578 nm in the experiment group were elevated with significant differences (P<0.05). Conclusions Low-level laser irradiation at 650 nm can decrease the hyperlipidemia erythrocyte absorption peaks and improve the balance between erythrocyte membrane and lipid profiles, which proves the feasibility of low-level laser for the treatment of hyperlipidemia from the perspective of spectroscopy. Key words: Low-level laser; Hyperlipidemia; Absorption spectrum; Multifunctional microplate reader