Infectibility of some new raspberry cultivars with arabismosaic and raspberry ringspot viruses and further evidence for variation in British isolates of these two nepoviruses

Abstract

SummaryIn field trials at sites of an outbreak of arabis mosaic nepovirus (AMV) in England and of raspberry ringspot nepovirus (RRV) in Scotland, the results of exposure of some new raspberry cultivars to natural infection with these viruses showed discrepancies from those obtained in graft inoculation tests using AMV‐Lib and RRV‐S, the Scottish type isolates. In particular, cv. Glen Prosen, which is immune to AMV‐Lib and RRV‐S, was infected with AMV and RRV in the field trials. Studies on these and other field isolates of AMV and RRV showed that they differed from the type isolates in Rubus host range and in symptomatology in herbaceous hosts. However, whereas four isolates of RRV found infecting Rubus were distinguishable by spur formation in gel double‐diffusion serological tests, six AMV isolates were indistinguishable by this method. Immunoelectrophoresis of virus particles did not distinguish the six AMV isolates, but isolates RRV‐MX and RRV‐T were distinguishable from RRV‐S and the English type isolate, RRV‐E. Like the two RRV type isolates, RRV‐MX contained a single electrophoretic component, but it migrated must faster whereas RRV‐T contained two components, one with a migration rate similar to that of RRV‐MX and the other similar to that of the type isolates. Polyacrylamide gel electrophoresis of protein preparations from highly purified virus particles of RRV isolates E, S and MX detected a single polypeptide of estimated mol. wt 54 × 103, 54 × 103 and 50 × 103 respectively but that of isolate T contained two polypeptides of estimated mol. wt 54 × 103 and 50 × 103. These data suggest that RRV‐T is a mixture of two isolates. In laboratory tests the nematode Xiphinema diversicaudatum transmitted four isolates of AMV efficiently whereas two populations of the nematode Longidorus elongatus were less efficient vectors of four RRV isolates. Neither vector species transmitted virus to any of nine raspberry cultivars. The results are discussed in relation to the control of nepoviruses in raspberry and to the biology of these viruses.