Abstract
PurposeTo investigate the effects of induction of selective liver hypothermia in a rodent model.MethodsSeven male Wistar rats were subjected to 90 minutes of partial 70% liver ischemia and topic liver 26°C hypothermia (H group). Other seven male Wistar rats were subjected to 90 minutes of partial 70% normothermic liver ischemia (N group). Five additional rats underwent a midline incision and section of liver ligaments under normothermic conditions and without any liver ischemia (sham group). All animals were sacrificed 24-h after reperfusion, and livers were sampled for analyses. Pathology sections were scored for sinusoidal congestion, ballooning, hepatocelllular necrosis and the presence of neutrophilic infiltrates.ResultsAt the end of the experiment, liver tissue expressions of TNF-ɑ, IL-1β, iNOS and TNF-ɑ/IL-10 ratio were significantly reduced in the H group compared to N group, whereas IL-10 and eNOS were significantly increased in H group. Histopathological injury scores revealed a significant decrease in ischemia/reperfusion (I/R) injuries in H group.ConclusionSelective liver hypothermia prevented I/R injury by inhibiting the release of inflammatory cytokines, preserves microcirculation, prevents hepatocellular necrosis and leukocyte infiltration, allowing maintenance of the liver architecture.
Highlights
Ischemia/reperfusion (I/R) injury remains one of the major problems in liver surgery and transplantation[1]
There was no significant difference between sham-operated controls (100 ± 5.6 mmHg), animals subjected to normothermic ischemia (89.7 ± 4.0 mmHg) and animals subjected to hypothermia (94.8 ± 8.3 mmHg) (P=0.554)
The present study is a continuation of our previous work, which demonstrated that selective liver hypothermia applied by topical cooling offers protection against I/R injuries in a 2-hour reperfusion model[12]
Summary
Ischemia/reperfusion (I/R) injury remains one of the major problems in liver surgery and transplantation[1]. Liver tolerance to oxygen deprivation spans 45-60 minutes, depending on the presence of steatosis or chronic liver disease This range may be extended to 120 minutes when liver hypothermia is induced[3]. The main technique for induction of hypothermia involves supra- and infrahepatic caval clamping, cannulation of portal vein trunk, infusion of chilled preservation solution and blood venting by a small orifice made either in the vena cava or suprahepatic veins. This technique is known as total vascular hepatic exclusion (TVHE) with in situ hypothermic perfusion. If proven as an effective practice, TH could serve as a tool to prevent hepatocellular damage
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