Abstract
Summary Succinic acid-2,2-dimethylhydrazide (B-Nine) induced nitrate reductase (NR) activity in detached, etiolated cucumber cotyledons as well in light as in darkness. Optimal concentration of B-Nine for NR induction was 25 mM. The compound inhibited growth and chlorophyll synthesis in the cotyledons. Chloramphenicol (CAP) and benzylaminopurine (BAP) also induced measurable NR activity in the cotyledons grown in darkness. B-Nine when applied at sub-optimal concentrations produced a synergistic effect with BAP and CAP: e.g. CAP (5 mM), B-Nine (5 mM) and CAP + B-Nine treated cotyledons produced about 4, 2 and 30 nmoles NO2− h−1 cotyledon−1, respectively. KNO3 did not potentiate effectivity of B-Nine or CAP as inducers of NR. Since cycloheximide reduced NR activity in the B-Nine and CAP treated cotyledons, it was inferred that the enzyme was synthesized de novo in cytoplasma. KNO3, BAP and B-Nine induced NADH-dependent NR (EC 1.6.6.1); B-Nine induced also some activity of NADPH-NR which was not detected in BAP + KNO3 induced cotyledons.
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