Abstract
A hydrogen bacterium, Alcaligenes hydrogenophilus, was grown aerobically under heterotrophic conditions. Cells were resuspended with nitrogen-free mineral salt medium and incubated under a gas mixture of H2, O2, and CO2.Membrane-bound and soluble hydrogenases could be induced in the non-proliferating cells after certain lag periods.Compounds supporting rapid growth strongly repressed the hydrogenases induction in the non-proliferating cells. It was also clarified by the method that carbon dioxide affected only the induction of soluble hydrogenase. Thus, this method enables us to analyze the effect of the component, which is indispensable for growth, on the induction.
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