Abstract
Lignocellulosic wastes are generally produced in huge amounts worldwide. Peach waste of these obtained from fruit juice industry was utilized as the substrate for laccase production by Pleurotus eryngii under solid state bioprocessing (SSB). Its chemical composition was determined and this bioprocess was carried out under stationary conditions at 28 °C. The effects of different compounds; copper, iron, Tween 80, ammonium nitrate and manganese, and their variable concentrations on laccase production were investigated in detail. The optimum production of laccase (43,761.33 ± 3845 U L−1) was achieved on the day of 20 by employing peach waste of 5.0 g and 70 µM Cu2+, 18 µM Fe2+, 0.025% (v/v) Tween 80, 4.0 g L−1 ammonium nitrate, 750 µM Mn2+ as the inducers. The dye decolorization also researched to determine the degrading capability of laccase produced from peach culture under the above-mentioned conditions. Within this scope of the study, methyl orange, tartrazine, reactive red 2 and reactive black dyes were treated with this enzyme. The highest decolorization was performed with methyl orange as 43 ± 2.8% after 5 min of treatment when compared to other dyes. Up to now, this is the first report on the induction of laccase production by P. eryngii under SSB using peach waste as the substrate.
Highlights
Laccases (E.C. 1.10.3.2) secreted by most of white rot fungi (WRF), are ligninolytic enzymes belonging to the multicopper oxidases containing four copper atoms in the catalytic center (Bertrand et al 2013)
The synthesis and activity of extracellular laccase are dependent on a number of factors that include the composition of the culture medium such as the nitrogen and/or carbon source and whether the organism is cultured in solid state bioprocessing (SSB) or fermentation systems (Singh and Chen 2008)
Many agroindustrial wastes were tested for laccase production, but peach waste (PW) has not been reported in production of laccase enzymes by fungi as per the published literature so far to the best of our knowledge
Summary
Laccases (E.C. 1.10.3.2) secreted by most of white rot fungi (WRF), are ligninolytic enzymes belonging to the multicopper oxidases containing four copper atoms in the catalytic center (Bertrand et al 2013) These enzymes are generally extracellular and catalyze the oxidation of several aromatic amines, phenolic compounds, thiols and some inorganic compounds using molecular oxygen as electron acceptor (Madhavi and Lele 2009). Reactive dyes are use generally in the dyeing industries due to their effective solubility, superior to color fastness properties to the applied fabric, high photolytic stability, and resistance to microbial deterioration (Spadaro et al 1992) Their degradations are of great importance in terms of ecological system. Considering the high volumes of these enzymes used mentioned industries, the cost of laccases productions is one of the main factors determining
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