Abstract
Tobacco (Nicotiana tabacum L. cv. Wisconsin 38) floral buds with sepal primordia only will continue organ production in a normal manner when excised from the plant and placed on the surface of a simple defined medium lacking growth substances. Such a medium is said to be developmentally inert because it does not induce or direct developmental events in the explant but does permit completion of developmental instructions, if any, carried in the explant. If the medium is supplemented with 10.0 ppm kinetin the bud produces a large but variable number of structures having some characteristics of sepals and some characteristics of petals and then continues with the initiation of fairly normal stamens and then carpels. This paper describes these structures and discusses the effect of kinetin on the regulatory systems controlling floral organ generation.
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