Abstract
Methanol dehydrogenase (MDH), is used by methanotrophic and methylotrophic bacteria to oxidize methanol to formaldehyde. (1) The enzyme is thought to exist both in a soluble form and in association with membrane. (2) Antisera prepared againstMethylomonas albusBG8 MDH andMethylobacteriumsp. strain AM1 MDH was used in an indirect Immunogold procedure to demonstrate localization of MDH. Following a modification of the method of De Mey (3), thin sections were incubated with primary antibody before exposure to gold-labeled secondary antibody.BG8, an obligate methanotroph, grows on methane and converts it to methanol. Methanol is then further oxidized by MDH to formaldehyde. A distinguishing feature of BG8 are extensive Intracytoplasmlc membranes (ICM) that form bundles or vesicular stacks located away from the peripheral membranes and extending into the cytoplasm (Fig. 1). (4) It is theorized that MDH is associated with ICM.
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