Abstract
A continuous-precipitation flow injection system coupled on-line with atomic absorption spectrometry for the determination of flavone in bee chrysalis has been developed. A complex precipitate was formed by injecting alkaline lead acetate into bee chrysalis samples and was retained on a Perspex filter followed by elution with nitric acid solution. The extraction method for flavone by the combination of a cold-methanol and alkaline solution was studied. The chemical conditions and the FI parameters were studied in detail. The calibration curve was linear over the range 1 - 100 µg ml-1. The detection limit was 0.6 µg ml-1. The relative standard deviation was 2.8% (n=9, 10 µg ml-1) and the recovery was 95.8 - 104%.
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