Abstract
The identification of interacting biomolecules in surface plasmon resonance (SPR) measurements can be achieved by attaching small characteristic covalent fluorophores. To implement this technique we studied the SPR response in the vicinity of an absorption band of dye molecules in ethanol and aqueous solutions. It is shown that the indirect determination of the absorption is possible from changes it induces in the SPR characteristics. Another technique studied employs the direct monitoring of the fluorescence signal in the surface plasmon field. The fluorescence light is then collected in the flow cell near the surface of a gold film with an optical fiber and transmitted to the registering photomultiplier. A significant enhancement of the fluorescent signal due to the excitation in the localized surface plasmon field was observed.
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