Abstract
Systemic damage of the organs, including the thyroid gland, is one of the key factors in the pathogenesis of burn disease due to thermal skin burns. The aim of this study was to investigate the indices of the cell cycle and DNA fragmentation of thyroid gland cells in rats with the use of infusion of 0.9% NaCl solution against the background of thermal skin burns. Experimental studies were conducted on 60 white male rats weighing 160-180 g, which was subjected to thermal burns of the skin of 2-3 degrees with a total area of 21-23% of the body surface. The first 7 days rats were infused with 0.9% NaCl solution into the inferior vena cava. Animals were removed from the experiment by decapitation (after 1, 3, 7, 14, 21, and 30 days). DNA content in the nuclei of the cells of the thyroid gland of rats was determined by flow cytometry. The statistical processing of the obtained results was carried out in the license package “STATISTICA 6.1” using nonparametric estimation methods. After 1 day after thermal skin damage and using 0.9% NaCl solution, lower (p<0.05) values of the S-phase index (0.234±0.094) were found compared to the control group without burn (0.652±0.134). The maximum decrease (p<0.01) of S-phase indicators (0.622±0.110 and 0.214±0.105, respectively) and a significant increase (p<0.01) of the SUB-G0G1 interval (5.288±0.840) compared to similar control group values (2.594±0.628) is observed after 3 days. The S-phase against the background of the introduction of 0.9% NaCl solution and thermal skin burn remained significantly lower than those of the similar control groups at 7 (p<0.01), 14 (p<0.05) and 21 days (p<0.05). At 14 days after thermal skin injury, the SUB-G0G1 interval (p<0.05) was lower than in the control group of rats. After 30 days, the G0G1 phase parameters were significantly lower (p<0.01), and the G2+M phase values were significantly (p<0.01) higher than those in the control group at the same time. Thus, it was found that 0.9% NaCl solution was not effective enough to correct cell division disorders during the entire observation period after skin burns.
Highlights
The urgency of the problem of therapy of thermal burns of the skin and burn disease (BD) is caused by the increase in the number of burn injuries in modern society, the lack of efficiency of existing methods of therapy, the high frequency of development of complications of a systemic nature [14]
The aim of the study was to investigate the indices of the cell cycle and DNA fragmentation of the thyroid gland in rats using infusion of 0.9% NaCl solution against the background of thermal skin burns
It is established that the background of the introduction of 0.9% NaCl solution 1 day after thermal burns of the skin marked changes in the cell cycle indices of the thyroid gland - statistically significant decrease in the number of cells in phase S (p
Summary
The urgency of the problem of therapy of thermal burns of the skin and burn disease (BD) is caused by the increase in the number of burn injuries in modern society, the lack of efficiency of existing methods of therapy, the high frequency of development of complications of a systemic nature [14]. The inefficiency of the proposed methods of treatment is not least due to the complex pathogenesis of this damage, the numerous factors responsible for the cascade of pathological processes in thermal burns [13] For this reason, the worldwide study of the mechanisms and pathogenetic factors of BD at the tissue, cell, subcellular and molecular levels perform, which deepens knowledge about this process and identifies potential targets of therapy [3]. A special role in the pathogenesis of BD according to modern data [18] is given to the interaction of the triangle pituitary gland - adrenal cortex, pituitary gland - thyroid gland It is known [8] that patients with severe thermal burns of the skin have decreased plasma triiodothyronine concentrations, low thyroxine and normal range, or slightly decreased thyrotropic hormone concentrations. Thyroid injury pathogenetically is associated with the level of endogenous intoxication and the development of general inflammatory response, established a clear link between changes in thyroid hormone metabolism and activation of various proinflammatory cytokines [15]
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