Abstract
According to the WHO, the thermal trauma is on the third place among other injuries. Burned injury is not only damage to the skin, but also the traumatization of all organs and systems of the body as a result of the stress response of the vascular system and the effects of toxic products coming from the area of burn injury. Firstly, such damages affect cardiomyocytes and the microcirculation vessels of the heart. The purpose of our study was to evaluate the changes in the cell cycle of myocardial cells in the left ventricle of rats under conditions of blood substitutes infusion and in the correction of experimental burn injury with a 0,9% solution of NaCl. The burn trauma was modeled using the Regas’ method and placed a catheter into the lower vena cava for intravenous infusion. The following solutions were used for infusion: 0,9% NaCl solution, lactoproteinum with sorbitol (Lactoproteinum-C) and colloidal-hyperosmolar HAES-LX-5% solution. Flow cytometry of the nuclear suspension of left ventricular cardiomyocytes was performed on the 1st, 3rd, and 7th days of the experiment. The statistical analysis of the results was carried out using the “STATISTICA 6.1” program package. The results of the performed study show a fairly stable picture of cell cycle parameters in myocardial cells of animals without burn injury with a predominance, on the one hand, of cells present in the G0G1 phase and the presence of a certain balance between the processes of creation of nuclear DNA synthesis and apoptosis. Changes in the phase of cardiac myocyte cell cycle against the background of the thermal injury of the skin throughout the observation time indicate a prolonged, uncorrected cell cycle disorder and a lack of effective normalization on the background of the physiological solution usage in the first 7 days after burning trauma of the skin. The protective effect of HAES-LX-5% prevents over-strain of cells, as evidenced by the lower synthetic activity of nuclei of cardiomyocytes at all times of the experiment.
Highlights
A large burn injury causes significant hemodynamic and cardiodynamic changes that support the development of sepsis, multiple organ failure, and leads to death [8]
Cardiogenic stress is a sign of the acute phase of response, and the negative results of the treatment of thermal damage are associated, in particular, with severe cardiac dysfunction [11, 12, 19]
Literary data indicate that the problem of adequate use of infusion-transfusion solutions in the case of burn injury is far from the solution [2, 5, 14, 15, 18]. It concerns the changes in the parameters of the cardiomyocyte cell cycle, which are almost not covered in modern literature
Summary
A large burn injury causes significant hemodynamic and cardiodynamic changes that support the development of sepsis, multiple organ failure, and leads to death [8]. Literary data indicate that the problem of adequate use of infusion-transfusion solutions in the case of burn injury is far from the solution [2, 5, 14, 15, 18]. First of all, it concerns the changes in the parameters of the cardiomyocyte cell cycle, which are almost not covered in modern literature. The aim of our study was to evaluate changes in the cell cycle of myocardial cells in the left ventricle of rats under conditions of infusion of blood substitutes and correction of experimental burn injury by a 0.9% solution of NaCl
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