Abstract
Abstract The synthesis of total cellular proteins and amylase (EC 3.2.1.1) has been studied in rat parotid gland slices in relation to secretory kinetics. In unstimulated slices, incorporation of radioactive amino acids is maintained at a constant rate for at least 3 hours. In response to epinephrine (30 µm), there is a 50% increase in incorporation into trichloracetic acid-insoluble proteins and a greater than 2-fold rise of incorporation into amylase after 1 hour of incubation. Concurrently there is active secretion of amylase into the medium. If, during the subsequent decline in protein synthesis, epinephrine is again added, the incorporation again doubles but there is no change in the secretory pattern. Dibutyryl cyclic AMP (2 mm) stimulates incorporation to the same extent as epinephrine. Cycloheximide, which suppresses protein synthesis to less than 10% of the control rate, does not stimulate secretion, nor does it inhibit the epinephrine-stimulated secretory response. It is concluded that epinephrine and dibutyryl cyclic AMP stimulate protein synthesis directly and independently of their effect on secretory activity.
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