Increasing Tolerance Agrostis Stolonifera, Festuca Rubra, Brachycome Iberidifolia, Chrysanthemum Carinatum to Copper

Abstract

There were used biotechnological methods for stress-tolerant lines through tissue culture based in vitro selection. The authors developed technologies for obtaining plants Agrostis stolonifera L., Festuca rubra rubra, Brachycome iberidifolia Benth., Chrysanthemum carinatum Schousb. resistant to copper. It was carried on a previously investigation that these plants are very sensitive to copper. For each object of the study was determined a selective concentration of copper and developed selection scheme. The scheme selection of A. stolonifera and F. rubra rubra included two–three subcultivations on the medium for callus growth, one passage on the medium for shoot regeneration, and one passage on the medium for rooting. All media contained 150 mg/l copper. The scheme selection of B. iberidifolia and C. carinatum consists of culturing callus on medium with addition a concentration of copper 20 mg/l during two passages, regeneration, and rooting on mediums without toxicant.