Increasing Temperatures Potentiate the Damage of Rare Earth Element Yttrium to the Crop Plant Triticum aestivum L

Abstract

Given that increasing temperature may aggravate the toxicity of pollutants, it is a daunting challenge to evaluate the realistic risks of rare earth elements (REEs) under global warming. Here, we studied how elevated temperatures (27 and 32 °C) impact the effect of yttrium (Y) on wheat plants (Triticum aestivum L.) at concentrations not causing effects (0, 0.5, and 1 μM) at the control temperature (22 °C) in a hydroponic system. After 14 days of exposure, significant inhibition (p < 0.05, 29.5%) of root elongation was observed only at 1 μM of Y at 32 °C. Exposure to Y at 27 °C showed no visible effects on root length, but induced significant (p < 0.05) metabolic disorders of a range of carbohydrates and amino acids related to galactose, phenylalanine, and glutamate metabolisms. Such cases were even shifted to substantial perturbation of the nucleotide pool reallocation involved in the disruption of purine and pyrimidine metabolism at 32 °C. These observations were regulated by sets of genes involved in these perturbed pathways. Using weighted gene co-expression network analysis, the disorder of nucleotide metabolism was shown to be responsible for the aggravated Y phytotoxicity at the extreme high temperature. Although the temperature fluctuation considered seems to be in an extreme range, unexpected implications driven by high temperature cannot be neglected. Our findings thus reduce the gaps of knowledge in REE toxicity to plants under future climate warming scenarios and highlight the importance of incorporating environmental temperature into the framework of the risk assessment of REEs.