Abstract
AbstractThe objective of this work was to synthesize a structured lipid (SL) enriched in stearidonic acid (SDA, C18:4 ω‐3), from modified soybean oil (MSO) originally containing ~25% SDA. Low temperature crystallization (LTC) of MSO triacylglycerols (TAG) and free fatty acids (FFA) was performed. The TAG and FFA crystallization products (LTC‐TAG and LTC‐FFA, respectively) had SDA contents of 48.72 and 60.78%, respectively. Enzymatic acidolysis between MSO and LTC‐FFA was studied utilizing Novozym 435 and Lipozyme TL IM as biocatalysts. Substrate molar ratio, incubation time, solvent, and enzyme load were explored. Equilibrium was reached at 96 and 48 h for Novozym 435 and Lipozyme TL IM‐catalyzed reactions, respectively. The best conditions from these studies were also applied to the acidolysis of LTC‐TAG and LTC‐FFA. Utilizing Lipozyme TL IM and solvent free conditions, SLs with SDA contents of 37.61 ± 1.00% (20.86 ± 6.48% at sn‐2 position) and 53.46 ± 1.85% SDA (36.37 ± 3.14% at sn‐2 position) were obtained from the acidolysis reaction between MSO and LTC‐FFA, and LTC‐TAG and LTC‐FFA, respectively. Compared to the original SDA content of MSO, this process leads to a 52 and 116% increase in SDA content, respectively.
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