Abstract
Heme oxygenase is an enzyme having three isoforms and is responsible for breakdown of heme into iron, carbon monoxide and bilirubin. There is no study related to heme oxygenase levels in human vitreous. So in this study our objectives were to measure heme oxygenase (HO) enzyme activity in vitreous and plasma of patients with proliferative diabetic retinopathy (PDR) and compare with the HO levels of patients with macular hole (MH). All protocols were approved by the Institution’s Ethics Board. Blood and vitreous samples were collected from 15 patients with PDR and 11 from macular hole who underwent vitrectomy surgery. HO enzyme activity was measured in vitreous and plasma samples by spectrophotometric method. In the PDR group, vitreous HO was 8.03 ± 1.95 pmol of bilirubin/mg protein which is significantly higher when compared to the MH group, 0.67 ± 0.2 pmol of bilirubin/mg protein. However, there was no difference in their HO activity in plasma, between the groups. Total Heme oxygenase enzyme activity is elevated in vitreous of PDR. Vitreous HO activity positively correlated with already reported adiponectin levels in these patients.
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