Abstract
A recently developed technique for determining the ploidy of fungal cells has been used to examine the relative ploidy levels of culture collection strains of two xylose-fermenting yeasts Candida shehatae and Pichia stipitis and their respective R strains. The R strains have been shown to have increased ploidy by at least a factor of two during their recycling in stressing media, namely wood hydrolysates. Their improved efficiency in xylose fermentation may be attributed at least in part to their increased ploidy.
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