Abstract
SummaryRabbit PBL were shown to incorporate predictably high levels of 3H-TdR following stimulation with PHA. The conditions necessary for optimal stimulation of rabbit PBL were: (a) separation of lymphocytes from whole blood by the Hypaque-Ficoll technique; (b) 2 × 106 PBL per culture in RPMI-1640 containing 5–10% heat-inactivated, autologous plasma; (c) 48 hr culture period; (d) 5 μg/ml PHA-P; and (e) 1 μCi/ml 3H-TdR added to cultures 22 hr before termination. Under these conditions, individual and group variations in the response to PHA were slight, and PBL responsiveness to other mitogens as well as to antigen were good.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
