Abstract

A laboratory bioreactor (1 L working volume) operating in chemostat mode was utilised for the culture of the polymorphic yeast Kluyveromyces marxianus var. marxianus NRRLy2415 undergoing fermentation of cheese whey permeate. Analysis of kinetic data revealed that the experimentally measured lactose concentration profiles in the exit line from the chemostat varied when the inlet feed concentrations ranged from 5–20 g lactose L−1. Further analysis revealed that this was due to about 3.5% of the feed bypassing the mixing zone. This incomplete mixing occurred despite an agitation rate of 800 rpm, an aeration rate of 1 vvm and the presence of internal baffles in the bioreactor. Without allowing for incomplete mixing, the estimation of the apparent Ks value for the organism could be out by a factor of five, over the range of feed concentrations employed. © Rapid Science Ltd. 1998

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