Inclusion of sorghum (Sorghum bicolor (L). Moench) with protease and NSP-ase increases jejunal histomorphology and barrier function gene expression in broiler chickens

Abstract

The objective of this study was to investigate the effect of sorghum with or without protease and NSP-ase on performance, jejunal histomorphology, caecal short-chain fatty acids (SCFA), and gene expression of barrier function in the broiler chickens. A total of 240 males New Lohmann strain MB 202 broiler chicks (1-day-old) were randomly placed into 12 treatments with 4 replications, 5 birds per replicate for 35 d. The diets were a factorial combination of 3 levels of sorghum (0, 10, and 20%), 2 concentrations of protease (with and without protease), and 2 concentrations of NSP-ase (with and without NSP-ase). Results showed that villus height (VH) was improved (P < 0.05) and villus to crypt ratio (VH:CD) tended (P = 0.05) to increase with inclusion of sorghum. Inclusion of sorghum upregulated (P < 0.05) expression of the gene anti-inflammatory IL-13, and downregulated (P < 0.05) gene of toll receptor (TLR) (TLR-3 and TLR-4), pro-inflammatory (IL-18, IL-6, tumor necrosis factor-alpha (TNF-α) and tight junction zona occluding-1 (ZO-1). The result further indicated a decrease (P < 0.05) in the concentration of SCFA. The birds fed diets with protease had lower (P < 0.05) expression of gene TLR-4 and TLR-3, pro-inflammatory (IL-6 and TNF-α), and anti-inflammatory IL-10. Inclusion of sorghum markedly (P < 0.05) interacted with protease, increasing the total of SCFA, acetate, and VH in the broiler. The bird offered diets with NSP-ase had higher (P < 0.05) expression genes of pro-inflammatory IL-18 and TNF-α. Inclusion of sorghum and NSP-ase significantly (P < 0.05) interacted, upregulating gene of tight junction ZO-1 and anti-inflammatory IL-13 but downregulating gene TLR-3, and pro-inflammatory IL-6, while the SCFA production was decreased. Inclusion of sorghum in the diet with or without enzymes did not affect broiler performance (P > 0.05). In conclusion, a diet with sorghum improved jejunal histomorphology without negatively affecting growth performance. Additionally, supplementation with enzymes enhanced intestinal immune responses by up-regulating anti-inflammatory, and downregulating pro-inflammatory gene expression.