Abstract
1. 1. The effects of two chlorine disinfectants, calcium hypochlorite (HTH) and 3-chloro-4.4-dimclhyl-2-oxazolidinonc (Agent I), on the activity of thymidylate synthase have been investigated. 2. 2. Although bolh disinfectants inactivated the enzyme, the following differences were observed: 3. 3. When the two disinfectants were used at the same total chlorine concentration, the rate and extent of inactivation were greater with Agent I than HTH. 4. 4. The substrate dUMP partially protected thyrnidylate synthase from inactivation by Agent 1, but it did not appreciably protect against inactivation by HTH. 5. 5. Large changes in the ultraviolet spectrum of the enzyme occurred when it was treated with HTH, which suggests reactions with aromatic amino acid side chains; no spectra) changes occurred when thyrnidylate synthase was treated with Agent I. 6. 6.Bocking the sulfhydryl groups of thymidylate synthase with sulfhydryl reagents prevented the irreversible inactivalion of the enzyme by Agent 1, but not by HTH.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
