Abstract
In this study, a spectral-domain optical coherence tomography (SD-OCT) system was used for noninvasive imaging of the adult zebrafish brain. Based on a 1325 nm light source and two high-speed galvo mirrors, our SD-OCT system can offer a large field of view of brain morphology with high resolution (12 μm axial and 13 μm lateral) at video rate (27 frame/s). In vivo imaging of both the control and injured brain was performed using adult zebrafish model. The recovered results revealed that olfactory bulb, optic commissure, telencephalon, tectum opticum, cerebellum, medulla, preglomerular complex and posterior tuberculum could be clearly identified in the cross-sectional SD-OCT images of the adult zebrafish brain. The reconstructed results also suggested that SD-OCT can be used for diagnosis and monitoring of traumatic brain injury. In particular, we found the reconstructed volumetric SD-OCT images enable a comprehensive three-dimensional characterization of the control or injured brain in the intact zebrafish.
Highlights
The brain is the most complex organ which acts as the center of the nervous system
Based on the 1325 nm spectral-domain optical coherence tomography (SD-optical coherence tomography (OCT)) system, our findings showed that the morphology of the whole adult zebrafish brain and associated functional brain structures could be clearly identified in vivo in the intact zebrafish
It was observed from the sagittal SD-OCT images in Fig. 2(b) that we could effectively distinguish among the different functional structures of the adult zebrafish brain
Summary
The brain is the most complex organ which acts as the center of the nervous system. The brain is very vulnerable to central nervous system diseases including Alzheimer's disease, Parkinson's disease and multiple sclerosis [1]. In the last two decades, zebrafish has attracted much attention in the investigation of brain as a model organism of vertebrate biology [2,3,4,5]. Histological sectioning and staining are the most commonly used methods to investigate the internal structures of the zebrafish brain These techniques need sacrifice the animals, which will bring significant challenges in studying the biological procedures in vivo and in dynamic processes. It is crucial to develop and use new optical imaging techniques that are able to achieve a high-resolution characterization of the adult zebrafish brain in the large field of view in terms of good penetration depth, fast imaging speed and high imaging sensitivity
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