Abstract
The aim of the current study was to monitor the migration of superparamagnetic iron oxide nanoparticle (SPION)-labeled C6 cells, which were used to induce glioblastoma tumor growth in an animal model, over time using magnetic resonance imaging (MRI), with the goal of aiding in tumor prognosis and therapy. Two groups of male Wistar rats were used for the tumor induction model. In the first group (n=3), the tumors were induced via the injection of SPION-labeled C6 cells. In the second group (n=3), the tumors were induced via the injection of unlabeled C6 cells. Prussian Blue staining was performed to analyze the SPION distribution within the C6 cells in vitro. Tumor-inducing C6 cells were injected into the right frontal cortex, and subsequent tumor monitoring and SPION detection were performed using T2- and T2*-weighted MRI at a 2T field strength. In addition, cancerous tissue was histologically analyzed after performing the MRI studies. The in vitro qualitative evaluation demonstrated adequate distribution and satisfactory cell labeling of the SPIONs. At 14 or 21 days after C6 injection, a SPION-induced T2- and T2*-weighted MRI signal reduction was observed within the lesion located in the left frontal lobe on parasagittal topography. Moreover, histological staining of the tumor tissue with Prussian Blue revealed a broad distribution of SPIONs within the C6 cells. MRI analyses exhibit potential for monitoring the tumor growth of C6 cells efficiently labeled with SPIONs.
Highlights
Brain tumors are defined as masses of abnormal cells in the brain that grow and multiply uncontrollably
The rats were divided into 2 groups: the first group (n=3) received tumors induced via the injection of superparamagnetic iron oxide nanoparticle (SPION)-labeled C6 cells, and the second group (n=3, control group) received tumors induced via the injection of unlabeled C6 cells
magnetic resonance imaging (MRI) monitoring of tumor growth according to the detection of SPION-labeled and unlabeled C6 glioma cells was performed 14 and 21 days after implantation
Summary
Brain tumors are defined as masses of abnormal cells in the brain that grow and multiply uncontrollably. Brain tumors, as well as other types of cancer, can carry a poor prognosis according to the specific genetic mutations involved. The most common human brain tumor, the astrocytoma, can be further classified according to its degree of malignant transformation into astrocytoma, anaplastic astrocytoma, or glioblastoma multiforme. The latter, a densely vascularized solid tumor characterized by the proliferation of blood vessels, endothelial cell hyperplasia, and endothelial cell cytology[2], presents the highest degree of malignancy and can rapidly invade normal brain tissue to cause death within months. As brain tumors often carry poor prognoses, it is necessary to attempt to enhance the quality of life of brain tumor patients by pursuing the development of new therapies
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