In vivo laxative, anti-diarrheal, hepatoprotective and diuretic investigations of Sterculia diversifolia and its isolated compound.

Abstract

To investigated the laxative, anti-diarrheal, hepatoprotective and diuretic activity of Sterculia diversifolia and its isolated compounds. The laxative activity was studied by counting wet stools while anti-diarrheal activity was performed by measuring gastrointestinal tract motility. Hepatoprotective activity was studied by biochemical and histo-pathological analysis while diuretic activity was performed by urine collection protocol. Doses of 30 and 100 mg/kg of crude methanolic extract of Sterculia diversifolia (MESD) stem bark and leaves, significantly (P < 0.05, P < 0.01) produced wet feces in subjects pretreated with atropine while 8-hydroxyquercetin and dihydroquercetin showed highly significant (P < 0.001) results by increasing fecal weight and water contents without producing diarrhea. MESD stem bark and leaves also dose-dependently lowered diarrhea while 8-hydroxyquercetin and dihydroquercetin showed highly significant (P < 0.001) results by producing shaped stools in mice. MESD, 8-hydroxyquercetin and dihydroquercetin offered significant protection against histopathological changes in the liver. Diuretic activity of Crude MESD stem bark and leaves extracts shows highly significant diuretic effect while dihydroquercetin showed better results than 8-hydroxyquercetin. It is concluded that Sterculia diversifolia and its isolated compounds bears laxative, anti-diarrheal, hepatoprotective and diuretic effects.