Abstract
A total of eight isolates, including variants and natural recombinants of Autographa californicaM NPV (Ac M NPY) for which genetic markers or physical maps are known were tested for in vivo virulence, and maintenance of genetic characteristics after infection of Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae) and Heliothis virescens (F.) (Lepidoptera: Noctuidae) larvae. Only one of the isolates was more virulent to T. ni than the wild type; however it was less virulent to H. virescens . One recombinant (AR-66) exhibited low production of polyhedra in TN368 cells, which were used for production of the polyhedra. One variant (Ac M NPY-S1) with an insertion of DNA when compared with the Ac M NPVE2 variant was at least 9.2 times less virulent than the wild isolate. After one in vivo passage of Ac M NPY-S1, a new variant (Ac M NPY-S1a) was detected with a new Eco-R1 fragment of 2.17 Mdal and a corresponding loss of a 1.55-Mdal Eco-R1 fragment found in the parental type.
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