In vivo and in vitro hair growth-promoting effect of silver and iron nanoparticles synthesized via Blumea eriantha DC plant extract.

Abstract

Blumea eriantha DC is the Indian medicinal remedy, mainly distributed in the States of India. It possesses wide array of medicinal properties. To execute green synthesis approach for the preparation of silver and iron nanoparticles by using alcoholic Blumea ErianthaDC extract and to verify the biological potential of the prepared nanoparticles as a hair growth promoter. Extract was mixed with silver nitrate and ferric chloride for synthesis of silver and iron nanoparticles, respectively. Prepared nanoparticles were confirmed by UV, FT-IR, SEM, X-ray diffraction, and TEM. The qualitative and quantitative analysis was performed namely hair growth initiation, hair growth completion, hair length, hair weight, histopathological studies, skin thickness, and length of the hair follicle. The prepared nanoparticles were observed as a blend of spherical and irregular shape with an average particle size of 35nm. The transition of anagen hair follicles was observed in approximately 63.43 % of animals treated with Minoxidil, whereas 2% and 5% Blumea erianthasilver nanoparticles treated animal group exhibited 33.02% and 60.93%, respectively. The animal groups treated with 2% and 5% iron nanoparticles of Blumea Eriantha showed 44.09 and 38.89% in anagen induction, respectively, which suggest the hair growth-promoting potential of the extract of Blumea erianthaDC. Thus, it can be concluded that silver nanoparticles of Blumea Eriantha exhibit promising hair growth promoting activity.